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hcd46  (R&D Systems)


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    Structured Review

    R&D Systems hcd46
    Hcd46, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd46/Human+CD46+Antibody/pm41989164-105-20-22
    Average 92 stars, based on 12 article reviews
    hcd46 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Membrane:

    Article Title: Detection of Otosclerosis-Specific Measles Virus Receptor (Cd46) Protein Isoforms
    Article Snippet: .. Membrane was blocked in 5% (w/v) skim milk powder in Tris-buffered saline Tween (TBST: 10 mM Tris, 150 mM NaCl, 0.05% [w/v] Tween20; pH = 7.9), and proteins were detected with anti-CD46 (1 : 200; R&D Systems, Minneapolis, MN, USA) and anti- β -actin (1 : 1000; Sigma-Aldrich, Deisenhofen, Germany) antibodies in 5% (w/v) skim milk powder in TBST. .. The blot was washed three times with TBST, incubated with horseradish peroxidase (HRP)-labeled secondary donkey anti-goat (R&D Systems, Minneapolis, MN, USA) and goat anti-rabbit (Santa Cruz, Santa Cruz, CA, USA) antibody at a dilution of 1 : 1000, washed and visualized with Super Signal West Pico Chemiluminescent Substrate (Pierce, Rockford, IL, USA), and exposed to X-ray film (Kodak, London, UK).

    Saline:

    Article Title: Detection of Otosclerosis-Specific Measles Virus Receptor (Cd46) Protein Isoforms
    Article Snippet: .. Membrane was blocked in 5% (w/v) skim milk powder in Tris-buffered saline Tween (TBST: 10 mM Tris, 150 mM NaCl, 0.05% [w/v] Tween20; pH = 7.9), and proteins were detected with anti-CD46 (1 : 200; R&D Systems, Minneapolis, MN, USA) and anti- β -actin (1 : 1000; Sigma-Aldrich, Deisenhofen, Germany) antibodies in 5% (w/v) skim milk powder in TBST. .. The blot was washed three times with TBST, incubated with horseradish peroxidase (HRP)-labeled secondary donkey anti-goat (R&D Systems, Minneapolis, MN, USA) and goat anti-rabbit (Santa Cruz, Santa Cruz, CA, USA) antibody at a dilution of 1 : 1000, washed and visualized with Super Signal West Pico Chemiluminescent Substrate (Pierce, Rockford, IL, USA), and exposed to X-ray film (Kodak, London, UK).

    Blocking Assay:

    Article Title: Accelerating target deconvolution for therapeutic antibody candidates using highly parallelized genome editing
    Article Snippet: Secondary antibodies: anti-h-IgG APC (Jackson ImmunoResearch #109-136-098), Anti-human Fab fragment-HRP (Jackson Immunoresearch #109-036-006). .. Polyclonal antibodies for blocking experiments: anti-HLA-A (MyBioSource #MBS8245132), anti-HLA-B (MyBioSource #MBS2522514), anti-HLA-B (Nordic BioSite #LS-C308249), anti-B2M (Sino Biological #11976-RP02), anti-CD5 (R&D Systems #AF1636), anti-CD7 (R&D Systems #AF7579), anti-CD46 (R&D Systems #AF2005), anti-CD97 (R&D Systems #AF2529), anti-ICAM1 (Sino Biological #10346-T26), anti-B7-H3 (Sino Biological #11188-T24), anti-Endoglin (Sino Biological #10149-T26), anti-IGSF3 (Sino Biological #11290-T24). ..

    Incubation:

    Article Title: Modified viruses
    Article Snippet: .. Cells were washed and detached by using Versene (Gibco) and immediately incubated with phycoerythrin-conjugated antibodies anti-SLAM (FAB1642P; R&D Systems), anti-CD46 (FAB2005P; R&D Systems), and anti-nectin-4 (FAB2659P; R&D Systems), or with control isotype antibody (IC0041P; R&D Systems). .. After incubation for 1 hour at 4° C., cells were washed again, and fluorescence was measured in a FACSCanto flow cytometry system (BD Bioscience).

    Article Title: Modified viruses
    Article Snippet: .. FACS Analysis and Quantification of Cell Surface Molecules Cells were washed and detached by using Versene (Gibco) and immediately incubated with phycoerythrin-conjugated antibodies anti-SLAM (FAB1642P; R&D Systems), anti-CD46 (FAB2005P; R&D Systems), and anti-nectin-4 (FAB2659P; R&D Systems), or with control isotype antibody (IC0041P; R&D Systems). .. After incubation for 1 hour at 4° C., cells were washed again, and fluorescence was measured in a FACSCanto flow cytometry system (BD Bioscience).

    Control:

    Article Title: Modified viruses
    Article Snippet: .. Cells were washed and detached by using Versene (Gibco) and immediately incubated with phycoerythrin-conjugated antibodies anti-SLAM (FAB1642P; R&D Systems), anti-CD46 (FAB2005P; R&D Systems), and anti-nectin-4 (FAB2659P; R&D Systems), or with control isotype antibody (IC0041P; R&D Systems). .. After incubation for 1 hour at 4° C., cells were washed again, and fluorescence was measured in a FACSCanto flow cytometry system (BD Bioscience).

    Article Title: Modified viruses
    Article Snippet: .. FACS Analysis and Quantification of Cell Surface Molecules Cells were washed and detached by using Versene (Gibco) and immediately incubated with phycoerythrin-conjugated antibodies anti-SLAM (FAB1642P; R&D Systems), anti-CD46 (FAB2005P; R&D Systems), and anti-nectin-4 (FAB2659P; R&D Systems), or with control isotype antibody (IC0041P; R&D Systems). .. After incubation for 1 hour at 4° C., cells were washed again, and fluorescence was measured in a FACSCanto flow cytometry system (BD Bioscience).

    FACS:

    Article Title: Modified viruses
    Article Snippet: .. FACS Analysis and Quantification of Cell Surface Molecules Cells were washed and detached by using Versene (Gibco) and immediately incubated with phycoerythrin-conjugated antibodies anti-SLAM (FAB1642P; R&D Systems), anti-CD46 (FAB2005P; R&D Systems), and anti-nectin-4 (FAB2659P; R&D Systems), or with control isotype antibody (IC0041P; R&D Systems). .. After incubation for 1 hour at 4° C., cells were washed again, and fluorescence was measured in a FACSCanto flow cytometry system (BD Bioscience).



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    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an <t>anti-CD46</t> blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.
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    Image Search Results


    (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an anti-CD46 blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.

    Journal: bioRxiv

    Article Title: A novel Gorilla-derived oncolytic Adenovirus with natural selective replication in cancer cells

    doi: 10.64898/2026.02.26.708271

    Figure Lengend Snippet: (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an anti-CD46 blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.

    Article Snippet: For the evaluation of CD46 and CAR cell surface levels, 2-3 x 10 5 cells (MRC5, HUVEC, A549, NCI-H1299, NCI-H1975, NCI-H727) were collected and incubated with anti-human CD46 (1:50; mouse monoclonal; #12239-MM05, Sino Biological) or with anti-human CAR (1:50; rabbit monoclonal; #10799-R271, Sino Biological) for 30 min at 4°C.

    Techniques: Infection, Blocking Assay, Flow Cytometry, Expressing, Virus